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| Santos, Paulo Augusto Almeida; Paiva, Renato; Silva, Luciano Coutinho; Souza, Ana Cristina de; Santana, Marlucia Cruz de; Silva, Diogo Pedrosa Corrêa da. |
| The aim of this study was to evaluate the efficiency of vitrification and droplet vitrification for the cryopreservation of Hancornia speciosa shoot tips. The shoot tips were subjected to four different periods of exposure (15, 30, 45 and 60 min.) to plant vitrification solution 2 (PVS2) before plunging into liquid nitrogen. We evaluated the regrowth of H. speciosa shoot tips that were cryopreserved by the classical vitrification technique and by the droplet vitrification technique. Shoot tips were submitted to different periods of pre-culture (absence, 24 or 48h) in a medium containing 0.3 M sucrose prior to cryopreservation. With a PVS2 exposure period of 60 min., significant differences were observed between the two techniques used in this study with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.00.00.00-4 - ciências agrárias; 2.03.03.00-9 - fisiologia vegetal; Native species; In vitro conservation; Recalcitrant seeds; Plant genetic diversity conservation; Vitrification; Droplet vitrification Conservação in vitro; Criopreservação vegetal. |
| Ano: 2015 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19573 |
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| Urrea Trujillo,Aura Inés; Botero Giraldo,Catalina; Naranjo Gómez,Esther Julia. |
| Abstract Psychotria ipecacuanha is a medicinal plant endangered in Colombia, due to the indiscriminate exploitation suffered several decades ago for its therapeutic importance. Its great medicinal value is due to emetine production and its derivatives, mainly in the roots. Given these concerns, the aim of this research was to evaluate different parameters for in vitro conservation of P. ipecacuanha by a limited plant growth, using as in vitro explants plant nodes. Plant growth response to 18, 23 and 25°C with and without addition to MS basal culture medium 15 and 30 gL-1 of mannitol osmotic regulator was evaluated. Registration of plant survival data (%), plant height (cm), plant development of axillary shoots, number of leaves, callus formation and vigour... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In vitro conservation; Plant limited growth; Osmotic regulator; Recovery phase; Vigour. |
| Ano: 2017 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000400598 |
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| Oliveira,Roberto Pedroso de; Silva,Sebastião de Oliveira e; Silva,Keuder Magalhães da; Silveira,Daniela Garcia. |
| A protocol for the in vitro conservation of diploid banana accessions based on lower temperature for culture environment was developed. Twenty four genotypes, four clones per genotype were studied. After disinfection, shoot tips (0.5 cm diameter x 0.5 cm height) were established in vitro and submitted to multiplication induced by benzylaminopurine. Twelve explants (0.6 cm diameter x 1.2 cm height) from each accession were transferred to the MS medium supplemented with 20 g L-1 sucrose and 2 g L-1 Phytagel, without growth regulators, under light intensity of 2000 lux, 16-hour photoperiod, at temperatures of: 17 ± 2ºC, 22 ± 2ºC and 26 ± 2ºC. The development of plantlets was evaluated during 450 days and the survived plantlets were acclimated under greenhouse... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Musa spp.; Germplasm; In vitro conservation. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000200008 |
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| Pacheco,Georgia; Gagliardi,Rachel Fátima; Cogliatti,Mariana Buturini; Manhães,Harlen Barreira; Carneiro,Leonardo Alves; Valls,José Francisco Montenegro; Mansur,Elisabeth. |
| The objective of this work was to evaluate the influence of substrate and preconditioning treatments on the acclimatization of in vitro plants of Arachis retusa. Plants were transferred to Plantmax or sand, and fertilized with Hoagland's nutrient solution. Plants maintained in sand, with or without fertilizer, showed the highest survival rates. In order to evaluate the influence of in vitro preconditioning treatments, stem segments were cultured on MS medium supplemented with different sucrose concentrations. The highest survival and developmental rates were observed in plants from two accessions cultured on MS supplemented with 1.5% and 3% sucrose. Flowering and fruit production were observed after five months. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arachis; Groundnut; In vitro conservation; Micropropagation. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000100023 |
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| Kavand, Somayeh; Agricultural Biotechnology Research Institute of Iran; Kermani, Maryam Jafarkhani; Agricultural Biotechnology Research Institute of Iran; Haghnazari, Ali; Agricultural Biotechnology Research Institute of Iran; Khosravi, Pegah; Agricultural Biotechnology Research Institute of Iran; Azimi, Mohamad Reza; Agricultural Biotechnology Research Institute of Iran. |
| In Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 µM BAP + 0 µM GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 µM BAP + 3 µM GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 µM... |
| Tipo: Laboratory analysis |
Palavras-chave: In vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta in vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279 |
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| Nogueira,Gabriela Ferreira; Pasqual,Moacir; Scherwinski-Pereira,Jonny Everson. |
| The objective of this work was to evaluate the phytotoxicity of a plant vitrification solution (PVS2), and the survival of shoot tips of the sugarcane variety SP716949, after cryopreservation by droplet-vitrification. Shoot tips were precultured for 24 hours in MS medium containing 0.3 mol L-1 sucrose, and exposed to PVS2 for 0, 20 or 30 min. Shoot tips were then immersed in liquid nitrogen. Thawing was fast in concentrated sucrose solution (1.2 mol L-1). PVS2 is a nontoxic to shoot tips, which in turn are sensitive to liquid nitrogen. The best results occurred when shoot tips were maintained for up to 20 min in PVS2 solution, before freezing, with 20% survival. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Saccharum; In vitro conservation; PVS2; Regeneration. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013001100014 |
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